ASP.NET: Working in old domain but ERR_CONNECTION_RESET in new Domain

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I have created a Blazor Server side Application which is working perfectly fine when accessed on a Win 2012 Server in our old Domain, no matter in which Browser.

If I host it on a Win 2019 Server in the new Domain though, it's only working in Internet Explorer. Edge displays the ERR_CONNECTION_RESET error. I did both of my tests from the same client which is located in the old Domain.

If I try to open it in Edge directly on the new Server, I receive another error: ERR_CONNECTION_CLOSED.

On both Servers it is running on Port 5001 (HTTPS)

Our Firewall department can't find any errors in the Logfiles. Hope you guys have got an idea what might help.

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