Convert pandas groupby dataframe into heatmap

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I'm trying to find a nice way to visualize the data from publicly available cBioPortal mutation data. I want to plot the co-occurrence of Protein Change (so basically, for each sample ID, does that specific sample have any other mutation also). See the image below:

cBio Portal Database DataFrame

I want to get this plotted as a heatmap (example below):

mutation heatmap

I've managed to get the data into the form of the first image above, but I am completely stuck as to how to go from there to the example heatmap.

I've looked into:

df.groupby(['Sample ID', 'Protein Change', 'Cancer Type Detailed']).count().unstack('Protein Change'))

which seems to go in the right direction, but not completely there.

Basically what I want is a heatmap with Protein Change on both axis, and a count of how many times those co-exist within a single sample.

Any help would be appreciated. Thanks!!

pandas-groupby pandas plotly heatmap python
2 Answers

You could do something like this:

Sample data:

df = pd.DataFrame({'Sample ID': [1, 1, 1, 4, 4, 5, 6, 6],
                   'Protein Change': ['A', 'B', 'C', 'D', 'A', 'C', 'A', 'B'],
                   'Cancer Type Detailed': 'Some type'})

   Sample ID Protein Change Cancer Type Detailed
0          1              A            Some type
1          1              B            Some type
2          1              C            Some type
3          4              D            Some type
4          4              A            Some type
5          5              C            Some type
6          6              A            Some type
7          6              B            Some type

Code:

import pandas as pd
import numpy as np
import seaborn as sns
import matplotlib.pyplot as plt

# Build co-occurrence matrix and set diagonal to zero.
ct = pd.crosstab(df['Sample ID'], df['Protein Change'])
co_occurrence = ct.T.dot(ct)
np.fill_diagonal(co_occurrence.to_numpy(), 0)

f, ax = plt.subplots(figsize=(4, 5))

# Mask lower triangular for plotting.
mask = np.tril(np.ones_like(co_occurrence))

cmap = sns.light_palette("seagreen", as_cmap=True)
sns.heatmap(co_occurrence, mask=mask, cmap=cmap, square=True, cbar_kws={"shrink": .65})
plt.show()

Result:

enter image description here

user_22468490

Does this works for you?

from itertools import combinations
# create a dataframe with all combinations for each Sample ID
df_combinations = df.groupby('Sample ID').agg({'Protein Change': lambda s: list(combinations(s, 2))})
# transform the obtained dataset to a two column format
df_combinations = pd.DataFrame(df_combinations['Protein Change'].explode().dropna().tolist(), columns=['Protein Change A', 'Protein Change B'])
# count how many times each combination appears
df_counts = df_combinations.groupby(['Protein Change A', 'Protein Change B']).size().to_frame('count').reset_index()
# pivot the df to obtain the desired matrix
df_counts.pivot(index='Protein Change A', columns='Protein Change B', values='count').fillna(0)
user_137656690
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