Python3 tkinter DLL load failed

I want to start a tkinter application with python3.9, but I get the following error:

>>> import tkinter as tk
Traceback (most recent call last):

  File "<ipython-input-18-e89bfb5dd03b>", line 1, in <module>
    import tkinter as tk

  File "C:\Program Files\Spyder\pkgs\tkinter\__init__.py", line 36, in <module>
    import _tkinter # If this fails your Python may not be configured for Tk

ImportError: DLL load failed: The specified module could not be found.

Is there a way to configure python3.9 for tkinter? I don't use cx_Freeze, but normal python interpeter.