Use order inside include in sequelize

Discussion.findAll({
    where : {
        discussion_on : profileId,
        is_deleted : false ,
        is_reply : false
    },
    limit : 5,
    order : [['updated_at','DESC']],
    offset:5*(page-1),
    // order : [['updated_at','DESC']],

    include:[{
        model : Profile,
        as : 'CommentBy',
        attributes:staticData.USER_LAYOUT,
        include : [{
            model : TeamMember,
            as : "TeamSender",
            required : false,
            attributes : ['receiver', 'title'],
            where : sequelize.where(sequelize.col('receiver'),db.sequelize.col('discussion.comment_entity'))
        },{
            model : TeamMember,
            required : false,
            as : "TeamReceiver",
            attributes : ['sender', 'title'],
            where : sequelize.where(sequelize.col('CommentBy.TeamReceiver.sender'),db.sequelize.col('discussion.comment_entity'))
        }]
    },{
        model : Discussion,
        as : 'Replies',
        required : false ,
        order : [['Replies.created_at','ASC']],
        where : {
            is_deleted : false
        } ,
        include:[{
            model : Profile,
            as : "CommentBy",
            attributes:staticData.USER_LAYOUT,
            include : [{
                model : TeamMember,
                as : "TeamSender",
                required : false,
                attributes : ['receiver', 'title'],
                where : sequelize.where(sequelize.col('Replies.CommentBy.TeamSender.receiver'),db.sequelize.col('Replies.comment_entity'))
            },{
                model : TeamMember,
                required : false,
                as : "TeamReceiver",
                attributes : ['sender', 'title'],
                where : sequelize.where(sequelize.col('Replies.CommentBy.TeamReceiver.sender'),db.sequelize.col('Replies.comment_entity'))
            }]
        },{
            model:Profile,
            as : "CommentEntity",
            attributes:staticData.USER_LAYOUT
        }]
    },{
            model:Profile,
            as : "CommentEntity",
            attributes:staticData.USER_LAYOUT
    }]
})

Discussion is the object name. Basically this query fetches all the comments with their replies attached to each comment .I want the replies to be sorted by date as well.

This is what i want to achieve inside Replies . But it does not work

order : [['created_at','ASC']],