WARN:Authentication error: ntlm authorization challenge expected, but not found in Soap UI

I am trying to access WCF Webservice using Windows Authentication in Soap UI.

I am getting 401 unauthorized.

Following are the set up I done.

1. In Request Authorization Tab, I have given as NTLM

2. I have given username, password and Domain.

As well as WSS-Password Type: PasswordText or PasswordDigest, and Wss-TimeToLive: 2000

Tried for Basic and Kerberos as well. Getting 401 response ONLY..

Getting following in the http log.

WARN:Authentication error: ntlm authorization challenge expected, but not found

What needs to be done to solve this issue?