Does an entire DICOM file has just one transfer syntax?

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Sorry if this is quite basic, I am new to DICOM. I know a DICOM file has multiple parts like: Patient, Study, Series and Instance (Image).

Now to communicate with a device it needs a Transfer Syntax, which tells the mode of communication, like Little-Endian, Big-Endian, JPEG-Lossless, lossy etc.

So, does each of the DICOM file parts (Patient, Study, Series and Instance (Image)) have their own transfer syntax? Like Patient can communicate as Little-Endian, Study might use JPEG-Lossless or MPEG-4 (if it is video) etc?

OR does the entire DICOM file just use one transfer syntax.

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