Python Database connection Close

You can define a DB class as below. Also, as andrewf suggested, use a context manager for cursor access.I'd define it as a member function. This way it keeps the connection open across multiple transactions from the app code and saves unnecessary reconnections to the server.

import pyodbc

class MS_DB():
    """ Collection of helper methods to query the MS SQL Server database.
    """

    def __init__(self, username, password, host, port=1433, initial_db='dev_db'):
        self.username = username
        self._password = password
        self.host = host
        self.port = str(port)
        self.db = initial_db
        conn_str = 'DRIVER=DRIVER=ODBC Driver 13 for SQL Server;SERVER='+ \
                    self.host + ';PORT='+ self.port +';DATABASE='+ \
                    self.db +';UID='+ self.username +';PWD='+ \ 
                    self._password +';'
        print('Connected to DB:', conn_str)
        self._connection = pyodbc.connect(conn_str)        
        pyodbc.pooling = False

    def __repr__(self):
        return f"MS-SQLServer('{self.username}', <password hidden>, '{self.host}', '{self.port}', '{self.db}')"

    def __str__(self):
        return f"MS-SQLServer Module for STP on {self.host}"

    def __del__(self):
        self._connection.close()
        print("Connection closed.")

    @contextmanager
    def cursor(self, commit: bool = False):
        """
        A context manager style of using a DB cursor for database operations. 
        This function should be used for any database queries or operations that 
        need to be done. 

        :param commit:
        A boolean value that says whether to commit any database changes to the database. Defaults to False.
        :type commit: bool
        """
        cursor = self._connection.cursor()
        try:
            yield cursor
        except pyodbc.DatabaseError as err:
            print("DatabaseError {} ".format(err))
            cursor.rollback()
            raise err
        else:
            if commit:
                cursor.commit()
        finally:
            cursor.close()

ms_db = MS_DB(username='my_user', password='my_secret', host='hostname')
with ms_db.cursor() as cursor:
        cursor.execute("SELECT @@version;")
        print(cur.fetchall())

The most common way to handle connections, if the language does not have a self closing construct like Using in .NET, then you should use a try -> finally to close the objects. Its possible that pyodbc does have some form of automatic closing but here is the code I do just in case:

conn = cursor = None
try:
    conn = pyodbc.connect('DRIVER=MySQL ODBC 5.1 driver;SERVER=localhost;DATABASE=spt;UID=who;PWD=testest') 

    cursor = conn.cursor()  

    # ... do stuff ...

finally:
    try: cursor.close()
    except: pass
    try: conn.close()
    except: pass