Python: "Indentation Error: unindent does not match any outer indentation level"

I just can't figure out what's wrong with this...

#!/usr/bin/env python
#
#       Bugs.py
#       

from __future__ import division

# No Module!
if __name__ != '__main__': 
    print "Bugs.py is not meant to be a module"
    exit()

# App
import pygame, sys, random, math
pygame.init()

# Configuration Vars
conf = {
    "start_energy": 50, 
    "food_energy": 25, 
    "mate_minenergy": 50, 
    "mate_useenergy": 35, 
    "lifespan": 300000
}

class Bugs:
    def __init__(self):
        self.list  = []
        self.timers= {}
        # Names / colors for sexes
        self.sex = ["Male", "Female"]
        self.color = ["#CBCB25", "#A52A2A"]
        # Bug info tracking
        self.bugid = 0
        self.buginfo = {"maxgen":0, "maxspeed":0}

    def new(self, x=False, y=False, sex=2, speed=0, generation=0, genes=[]):
        sex   = sex   if not sex   == 2 else random.randint(0,1)
        speed = speed if not speed == 0 else random.randint(1,3)
        # Create new bug object
        self.bugs.append(BugObj(sex, speed, generation, bugid, pygame.time.get_ticks, genes))
        # Make sure it has a timer
        if not self.timers[speed]:
            self.timers[speed] = 1
            pygame.time.set_timer(25 + speed, 1000 / speed)
        # Update info tracking variables
        if speed      > self.buginfo["maxspeed"]: self.buginfo["maxspeed"] = speed
        if generation > self.buginfo["maxgen"]  : self.buginfo["maxgen"]   = generation
        self.bugid += 1

    def speed_count(self, speed):
        a = 0
        for i in list[:]:
            if i.speed = speed:
                a += 1
        return a

class BugObj:
    def __init__(self, sex, speed, generation, bugid, born, genes):
        global conf
        self.sex        = sex
        self.speed      = speed
        self.generation = generation
        self.id         = bugid
        self.born       = born
        self.genes      = genes
        self.died       = -1
        self.energy     = conf["start_energy"]
        self.target     = "None"

    def update(self):
        global conf
        if self.age() > conf["lifespan"]:
            self.die()
        else:
            f = closest_food()
            m = closest_mate()
            # If there's a potential mate
            if m != 0 and self.energy > conf["mate_minenergy"]:
                if not self.rect.colliderect(m.rect):
                    self.move_toward(m)
                    self.target = "Mate: " + str(m.rect.center)
                else:
                    Bugs.mate(self, m)
                    self.target = "Mate: (Reached)"
            elif f != 0:
                if not self.rect.colliderect(f.rect):
                    self.move_toward(f)
                    self.target = "Food: " + str(f.rect.center)
                else:
                    self.eat(f)
                    self.target = "Food: (Reached)"
            else:
                self.target = "Resting"
            # Use energy
            self.energy -= 0

    def closest_food(self):
        pass

    def closest_mate(self):
        pass

    def age(self):
        if self.died != -1:
            return pygame.time.get_ticks - self.born
        else:
            return self.died - self.born

    def die(self):
        # Remove self from the list
        Bugs.list.remove(self)
        # Turn off timer
        if not Bugs.speed_count(self.speed):
            Bugs.timers[self.speed] = 0
            pygame.time.timers(25 + self.speed, 0)
        # Bye!
        del self

class Food:
    def __init__(self)
        pass

    def update(self)
        pass

# Update Loop
while 1:
    ev = pygame.event.wait()
    speed = ev.type - 25
    if speed > 24:
        for i in Bugs.list[:]:
            if i.speed = speed
                i.update()
                print "Updating bug #" + str(i.id)
    if speed == 0:
        Food.update()

I get the following every time:

  File "Bugs.py" line 53
    def new(self, x=False, y=False, sex=2, speed=0, generation=0, genes=[]):
                                                                           ^
Indentation Error: unindent does not match any outer indentation level