Limits in the context of Unicode multi-byte character capabilities
While RFC5322 defines a limit of 1000 (998 + CRLF) characters, it does so in the context of headers limited to ASCII characters only.
RFC 6532 explains how to handle multi-byte Unicode characters.
Section 3.4 ( Effects on Line Length Limits ) states:
Section 2.1.1 of [RFC5322] limits lines to 998 characters and
recommends that the lines be restricted to only 78 characters. This
specification changes the former limit to 998 octets. (Note that, in
ASCII, octets and characters are effectively the same, but this is
not true in UTF-8.) The 78-character limit remains defined in terms
of characters, not octets, since it is intended to address display
width issues, not line-length issues.
So for example, because you are limited to 998 octets, you can't have 998 smiley faces in your subject line as each emoji of this type is 4 octets.
Using PHP to demonstrate:
Run php -a for an interactive terminal.
// Multi-byte string length:
var_export(mb_strlen("\u{0001F602}",'UTF-8'));
// 1
// ASCII string length:
var_export(strlen("\u{0001F602}"));
// 4
// ASCII substring of four octet character:
var_export(substr("\u{0001F602}",0,4));
// ''
// ASCI substring of four octet character truncated to 3 octets, mutating character:
var_export(substr("\u{0001F602}",0,3));
// '▒'