I created heatmap with ggplot geom_tile(). For some reason the colors in the heatmap are not sorted as the values in column 'value' according to 'GFP_wide' in the variable column, (event that the values are already sorted). So my question is how do I make my heat map be sorted according to the variable GFP_wide? Thank you in advance!
I have the following data set:
GFP_long<-data.frame(Entrez.Symbol<-c("TRIP11","SIN3B","SF3B1","PSMD14","RAD51","BARD1",
"BRCA1","BRCA1","U5200KD","SETD5","TRIP11","SIN3B",
"SF3B1","PSMD14","RAD51","BARD1","BRCA1","BRCA1",
"U5200KD","SETD5"),
variable<-c("GFP_my","GFP_my","GFP_my","GFP_my","GFP_my","GFP_my","GFP_my",
"GFP_my","GFP_my","GFP_my","GFP_wide","GFP_wide","GFP_wide","GFP_wide",
"GFP_wide","GFP_wide","GFP_wide","GFP_wide","GFP_wide","GFP_wide"),
value<-c(1.015,0.336,0.315,0.254,0.286,0.157,0.275,0.230,0.772,0.364,
0.020,0.055,0.071,0.102,0.109,0.139,0.146,0.146,0.151,0.179))
colnames(GFP_long)<-c("Entrez.Symbol","variable","value")
So it looks like this:
Entrez.Symbol variable value
1 TRIP11 GFP_my 1.015
2 SIN3B GFP_my 0.336
3 SF3B1 GFP_my 0.315
4 PSMD14 GFP_my 0.254
5 RAD51 GFP_my 0.286
6 BARD1 GFP_my 0.157
7 BRCA1 GFP_my 0.275
8 BRCA1 GFP_my 0.230
9 U5200KD GFP_my 0.772
10 SETD5 GFP_my 0.364
11 TRIP11 GFP_wide 0.020
12 SIN3B GFP_wide 0.055
13 SF3B1 GFP_wide 0.071
14 PSMD14 GFP_wide 0.102
15 RAD51 GFP_wide 0.109
16 BARD1 GFP_wide 0.139
17 BRCA1 GFP_wide 0.146
18 BRCA1 GFP_wide 0.146
19 U5200KD GFP_wide 0.151
20 SETD5 GFP_wide 0.179
My ggplot code:
ggplot(GFP_long, aes(x=Entrez.Symbol,y=variable,fill=value)) +
geom_tile(aes(fill = value))+
scale_fill_gradient(low="red", high="green")
